Abstract
Purpose and Appropriate Sample Types
This panel was optimized to assess CD4+ and CD8+ T cell responses to various tumor antigens from melanoma patients. The panel was tested on single-cell derived T cell isolates (SCD-T) and T cell lines derived from peripheral blood mononuclear cells (PBMC) from melanoma patients, T cell lines from the tumor environment of melanoma patients, and fresh and cryopreserved PBMC (healthy donors). Staining can be performed in 96-well plates for high-throughput.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Antigens, Neoplasm / immunology
-
Blood Cells / cytology*
-
Blood Cells / immunology
-
CD8-Positive T-Lymphocytes
-
Case-Control Studies
-
Cryopreservation
-
Cytokines / analysis*
-
Cytokines / immunology
-
Flow Cytometry
-
Fluorescent Dyes
-
Humans
-
Immunophenotyping
-
Lymphocyte Activation / immunology
-
Melanoma / immunology*
-
Melanoma / metabolism
-
Melanoma / pathology
-
Primary Cell Culture
-
Th1 Cells / immunology*
-
Th1 Cells / metabolism
-
Th1 Cells / pathology
-
Th1-Th2 Balance
-
Th2 Cells / immunology*
-
Th2 Cells / metabolism
-
Th2 Cells / pathology
-
Tumor Microenvironment / immunology
Substances
-
Antigens, Neoplasm
-
Cytokines
-
Fluorescent Dyes