Phenolic acid composition and antioxidant properties of Malaysian honeys

J Food Sci. 2011 Aug;76(6):C921-8. doi: 10.1111/j.1750-3841.2011.02282.x.

Abstract

The phenolic acid and flavonoid contents of Malaysian Tualang, Gelam, and Borneo tropical honeys were compared to those of Manuka honey. Ferric reducing/antioxidant power assay (FRAP) and the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activities were also quantified. All honey extracts exhibited high phenolic contents (15.21 ± 0.51- 42.23 ± 0.64 mg/kg), flavonoid contents (11.52 ± 0.27- 25.31 ± 0.37 mg/kg), FRAP values (892.15 ± 4.97- 363.38 ± 10.57 μM Fe[II]/kg), and high IC₅₀ of DPPH radical-scavenging activities (5.24 ± 0.40- 17.51 ± 0.51 mg/mL). Total of 6 phenolic acids (gallic, syringic, benzoic, trans-cinnamic, p-coumaric, and caffeic acids) and 5 flavonoids (catechin, kaempferol, naringenin, luteolin, and apigenin) were identified. Among the Malaysian honey samples, Tualang honey had the highest contents of phenolics, and flavonoids, and DPPH radical-scavenging activities. We conclude that among Malaysian honey samples, Tualang honey is the richest in phenolic acids, and flavonoid compounds, which have strong free radical-scavenging activities.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis*
  • Antioxidants / chemistry
  • Carboxylic Acids / analysis*
  • Carboxylic Acids / chemistry
  • Chromatography, High Pressure Liquid
  • Flavonoids / analysis
  • Flavonoids / chemistry
  • Free Radical Scavengers / analysis
  • Free Radical Scavengers / chemistry
  • Honey / analysis*
  • Malaysia
  • Phenols / analysis*
  • Phenols / chemistry
  • Solid Phase Extraction
  • Spectrophotometry

Substances

  • Antioxidants
  • Carboxylic Acids
  • Flavonoids
  • Free Radical Scavengers
  • Phenols