CTCF-dependent chromatin insulator as a built-in attenuator of angiogenesis

Transcription. 2012 Mar-Apr;3(2):73-7. doi: 10.4161/trns.19634. Epub 2012 Mar 1.

Abstract

VEGF is a pivotal pro-angiogenic growth factor and its dosage decisively impacts vascularization. We recently identified a CTCF-dependent chromatin insulator that critically restrains the transcriptional induction of VEGF and angiogenesis. We postulate that CTCF may exert enhancer blocking by mediating chromatin looping and/or RNA polymerase pausing at the VEGF locus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • CCCTC-Binding Factor
  • Chromatin / metabolism*
  • Enhancer Elements, Genetic
  • Histones / metabolism
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Insulator Elements
  • K562 Cells
  • Neovascularization, Physiologic*
  • RNA Polymerase II / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • CCCTC-Binding Factor
  • CTCF protein, human
  • Chromatin
  • Histones
  • Repressor Proteins
  • Vascular Endothelial Growth Factor A
  • RNA Polymerase II