Quantitative analysis of cellular senescence phenotypes using an imaging cytometer

Miyako Udono; Keishi Kadooka; Shuntaro Yamashita; Yoshinori Katakura

doi:10.1016/j.ymeth.2012.02.012

Quantitative analysis of cellular senescence phenotypes using an imaging cytometer

Methods. 2012 Mar;56(3):383-8. doi: 10.1016/j.ymeth.2012.02.012. Epub 2012 Mar 3.

Authors

Miyako Udono¹, Keishi Kadooka, Shuntaro Yamashita, Yoshinori Katakura

Affiliation

¹ Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.

PMID: 22406489
DOI: 10.1016/j.ymeth.2012.02.012

Abstract

Until now, various stimuli as well as serial passaging have been known to induce cellular senescence in normal human diploid fibroblasts. However, in many cases, we have encountered difficulty in quantitatively analyzing the cellular senescence phenotypes of senescent cells in a physiological condition. High-content screening (HCS)-based image analysis is becoming an important and powerful research tool. In the present study, an automated and quantitative cellular image-analysis system was employed to quantify the cellular senescence phenotypes induced in normal human diploid fibroblasts, TIG-1 cells, and found to be a powerful tool in the cellular senescence study.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Cellular Senescence*
Humans
Image Cytometry / methods*
Phenotype