New enzyme-linked immunosorbent assay for detection of antibodies against hepatitis delta virus using a hepatitis delta antigen derived from a Taiwanese clone and comparison to the Abbott radioimmunoassay

Yung-Bin Kuo; Mei Chao; Yi-Hsuan Lee; Chau-Ting Yeh; Err-Cheng Chan

doi:10.1128/CVI.05687-11

New enzyme-linked immunosorbent assay for detection of antibodies against hepatitis delta virus using a hepatitis delta antigen derived from a Taiwanese clone and comparison to the Abbott radioimmunoassay

Clin Vaccine Immunol. 2012 May;19(5):817-9. doi: 10.1128/CVI.05687-11. Epub 2012 Mar 7.

Authors

Yung-Bin Kuo¹, Mei Chao, Yi-Hsuan Lee, Chau-Ting Yeh, Err-Cheng Chan

Affiliation

¹ Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan.

Abstract

An anti-hepatitis delta (HD) enzyme-linked immunosorbent assay (ELISA) using a specific recombinant hepatitis delta antigen derived from a local dominant hepatitis delta virus (hepatitis D virus; HDV) strain in Taiwan has been established. The detection efficiency of this assay was comparable to that of the commercially available Abbott anti-HD radioimmunoassay (RIA) and could be useful in routine laboratory diagnoses of HDV infection.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Clinical Laboratory Techniques / methods*
Enzyme-Linked Immunosorbent Assay / methods
Hepatitis Antibodies / blood*
Hepatitis D / diagnosis*
Hepatitis Delta Virus / immunology*
Hepatitis delta Antigens
Humans
Radioimmunoassay / methods
Recombinant Proteins
Taiwan

Substances

Hepatitis Antibodies
Hepatitis delta Antigens
Recombinant Proteins