Abstract
Group A rotavirus genotype G1P[8] is the most common strain affecting humans around the world over the past few decades. In this study, we examined genetic variation in the VP7 gene of rotavirus G1P[8] strains, detected in children of four major cities of Vietnam during three different rotavirus seasons: 1998-1999, 2007-2008 and 2008-2009 in order to assess the evolution of the virus over 11 years. Fecal samples (n=73) from children hospitalized for gastroenteritis caused by G1P[8] rotavirus were analyzed by DNA sequencing of gene 9 encoding the VP7 capsid protein. Phylogenetic analyses indicated that VP7 gene of the G1 strains from 1999 contained a lineage I, while rotaviruses from 2009 clustered in lineage II. Both of these lineages were found co-circulating in 2007-2008 season. While different sublineages of lineage I and II co-circulated in the 1998-1999 and 2007-2008 seasons, almost all strains in 2009 belonged to sub-lineage II-C. In the analysis using selected 10 strains, the VP4 genes of these 2 VP7-G1 lineages were all grouped in F45-like cluster. Deduced amino acid analyses indicated that there were thirteen amino acid substitutions between strains of two lineages. Of those, two were found in antigenic regions A and C, implying possible antigenic differences between these two lineages. The G1P[8] strains in Vietnam are very genetically diverse and dynamic, implying the frequent monitoring on evolution of rotavirus will be important to assess efficacy of rotavirus vaccine in Vietnam.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Viral / genetics*
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Capsid Proteins / genetics*
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Child, Preschool
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Cluster Analysis
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DNA, Viral / chemistry
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DNA, Viral / genetics
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Gastroenteritis / epidemiology
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Gastroenteritis / virology
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Genetic Variation*
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Humans
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Infant
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Infant, Newborn
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Molecular Epidemiology
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Molecular Sequence Data
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Phylogeny
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Rotavirus / genetics*
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Rotavirus / isolation & purification
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Rotavirus Infections / epidemiology*
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Rotavirus Infections / virology*
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Sequence Analysis, DNA
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Vietnam / epidemiology
Substances
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Antigens, Viral
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Capsid Proteins
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DNA, Viral
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VP7 protein, Rotavirus
Associated data
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GENBANK/JF690673
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GENBANK/JF690674
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GENBANK/JF690675
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GENBANK/JF690676
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