Abstract
A library of α2-3- and α2-6-linked sialyl galactosides containing C9-modified sialic acids was synthesized from C6-modified mannose derivatives using an efficient one-pot three-enzyme system. These sialosides were used in a high-throughput sialidase substrate specificity assay to elucidate the importance of C9-OH in sialidase recognition.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Proteins / chemistry*
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Galactosides / chemistry*
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High-Throughput Screening Assays
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Humans
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Mannose / analogs & derivatives
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Mannose / chemistry
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Neuraminidase / chemistry*
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Sialic Acids / chemistry*
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Sialyltransferases / chemistry*
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Substrate Specificity
Substances
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Bacterial Proteins
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Galactosides
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Sialic Acids
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Sialyltransferases
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Neuraminidase
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Mannose