Crystallography on a chip

Acta Crystallogr D Biol Crystallogr. 2012 Mar;68(Pt 3):321-3. doi: 10.1107/S0907444911055296. Epub 2012 Feb 14.

Abstract

A new chip-based crystal-mounting approach for rapid room-temperature data collection from numerous crystals is described. This work was motivated by the recent development of X-ray free-electron lasers. These novel sources deliver very intense femtosecond X-ray pulses that promise to yield high-resolution diffraction data of nanocrystals before their destruction by radiation damage. Thus, the concept of `diffraction before destruction' requires rapid replenishment of the sample for each exposure. The chip promotes the self-assembly of an array of protein crystals on a surface. Rough features on the surface cause the crystals to adopt random orientations, allowing efficient sampling of reciprocal space.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallography, X-Ray / instrumentation*
  • Crystallography, X-Ray / methods
  • Lasers
  • Nanoparticles / chemistry
  • Proteins / chemistry

Substances

  • Proteins