Biochemical methane potential tests were conducted to evaluate the effect of using a blank versus a pre-incubated inoculum in digestion of primary sludge at different waste to inoculum ratios (S/X). In addition, this study explored the influence of using two different anaerobic inoculum sources on the digestion of food waste: digested sludge from a municipal wastewater treatment plant and from a digester treating the organic fraction of municipal solid wastes. The results revealed that although there was no significant difference in methane yield (on average 114mLCH(4)/g TCOD(sub)) or biodegradability (on average 28.3%) of primary sludge using pre-incubated or non-incubated inocula, the maximum methane production rates using non-incubated inoculum were higher than those using pre-incubated inoculum at all S/X ratios. Moreover, interestingly the inoculum from an anaerobic digester treating municipal wastewater sludge was superior over the inoculum from anaerobic digester treating food waste in digesting food waste.
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