Algorithm proposal based on the C. Diff Quik Chek Complete ICT device for detecting Clostridium difficile infection

Enferm Infecc Microbiol Clin. 2013 Feb;31(2):97-9. doi: 10.1016/j.eimc.2012.01.003. Epub 2012 Feb 15.

Abstract

Objective: To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficile infection (CDI) based on this test.

Methods: We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference.

Results: This test enabled us to obtain a conclusive result in <30min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C. difficile by toxigenic culture.

Conclusion: This test has a high sensitivity and specificity for the diagnosis of CDI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Antigens, Bacterial / analysis
  • Azure Stains*
  • Chromatography, Affinity* / instrumentation
  • Clostridium Infections / diagnosis*
  • Glutamate Dehydrogenase / immunology
  • Humans
  • Methylene Blue*
  • Xanthenes*

Substances

  • Antigens, Bacterial
  • Azure Stains
  • Diff Quik
  • Xanthenes
  • Glutamate Dehydrogenase
  • Methylene Blue