Fructose-1,6-bisphosphate protects against Zymosan-induced acute lung injury in mice

Inflammation. 2012 Jun;35(3):1198-203. doi: 10.1007/s10753-012-9429-6.

Abstract

It has been previously showed that fructose-1,6-bisphosphate (FBP) has anti-inflammatory and immunomodulatory effects on several experimental inflammation models. However, the effects and mechanism of FBP on Zymosan-induced acute lung injury (ALI) in mice had not been tested. In this study, our aim was to assess the anti-inflammatory activities of FBP on Zymosan-induced ALI. We found that in vivo treatment with FBP (500 mg/kg i.p.) markedly decreased the nitric oxide (NO) levels in the lungs and significantly reduced bronchoalveolar lavage fluid total cell and neutrophil counts and protein exudation after Zymosan challenge. Furthermore, FBP inhibited inducible nitric oxide synthase (iNOS) activities in RAW macrophages. Meanwhile, FBP did not inhibit the cyclooxigenase 2, interleukin-6, and nuclear factor kappa B transcription. Taken together, these results suggest that FBP shows anti-inflammatory effects through inhibiting lung edema, NO, and iNOS activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / chemically induced
  • Acute Lung Injury / drug therapy*
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchoalveolar Lavage Fluid / cytology
  • Cyclooxygenase 2 / biosynthesis
  • Cyclooxygenase 2 / genetics
  • Edema / drug therapy
  • Edema / immunology
  • Edema / pathology
  • Fructosediphosphates / pharmacology*
  • Fructosediphosphates / therapeutic use
  • Inflammation / chemically induced
  • Inflammation / drug therapy
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / genetics
  • Lung / drug effects
  • Lung / metabolism
  • Lung / pathology
  • Macrophages / metabolism
  • Male
  • Mice
  • NF-kappa B / biosynthesis
  • NF-kappa B / genetics
  • Neutrophils
  • Nitric Oxide / metabolism*
  • Nitric Oxide Synthase Type II / biosynthesis*
  • Nitric Oxide Synthase Type II / metabolism
  • Transcription, Genetic
  • Zymosan

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Fructosediphosphates
  • Interleukin-6
  • NF-kappa B
  • Nitric Oxide
  • Zymosan
  • Nitric Oxide Synthase Type II
  • Cyclooxygenase 2
  • fructose-1,6-diphosphate