Direct patterning of mammalian cells in an ultrasonic heptagon stencil

Biomed Microdevices. 2012 Jun;14(3):559-64. doi: 10.1007/s10544-012-9633-z.

Abstract

We describe the construction of a ultrasonic device suitable for micro patterning particles and cells for tissue engineering applications. The device is formed by seven transducers shaped into a heptagon cavity. By exciting two and three transducers simultaneously, lines or hexagonal shapes can be formed with beads and cells. Furthermore, phase control of the transducers allows shifting the standing waves and thus patterning at different positions on a surface in a controlled manner. The paper discusses direct patterning of mammalian cells by ultrasound "stencil".

MeSH terms

  • Animals
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Equipment Design / instrumentation*
  • Equipment Design / methods
  • Mammals
  • Microtechnology / instrumentation
  • Microtechnology / methods
  • Polystyrenes / chemistry
  • Tissue Engineering / methods
  • Transducers
  • Ultrasonics / instrumentation*

Substances

  • Polystyrenes