Protective effects of aminoethyl-chitooligosaccharides against oxidative stress in mouse macrophage RAW 264.7 cells

Int J Biol Macromol. 2012 Apr 1;50(3):624-31. doi: 10.1016/j.ijbiomac.2012.01.036. Epub 2012 Feb 4.

Abstract

The aim of this study is to investigate the inhibitory effects of aminoethyl-chitooligosaccharides (AE-COS) on oxidative stress in mouse macrophages (RAW 264.7 cells). The inhibitory effects of AE-COS on DNA and protein oxidation were studied in RAW 264.7 cells. Furthermore, free radical scavenging effect of AE-COS were determined in RAW264.7 cells by 2',7'-dichlorofluorescein (DCF) intensity and intracellular glutathione (GSH) level. AE-COS also inhibited myeloperoxidase (MPO) activity in human myeloid cells (HL-60). These results suggest that AE-COS acts as a potential free radical scavenger in RAW 264.7 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Cytoprotection / drug effects*
  • DNA / genetics
  • DNA / metabolism
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Glutathione / metabolism
  • HL-60 Cells
  • Humans
  • Intracellular Space / drug effects
  • Intracellular Space / metabolism
  • Macrophages / cytology
  • Macrophages / drug effects*
  • Macrophages / enzymology
  • Macrophages / metabolism*
  • Membrane Proteins / metabolism
  • Mice
  • Oligosaccharides / chemistry*
  • Oligosaccharides / pharmacology*
  • Oxidation-Reduction / drug effects
  • Oxidative Stress / drug effects*
  • Peroxidase / antagonists & inhibitors
  • Structure-Activity Relationship

Substances

  • Free Radical Scavengers
  • Membrane Proteins
  • Oligosaccharides
  • DNA
  • Peroxidase
  • Glutathione