New cyclic peptides with osteoblastic proliferative activity from Dianthus superbus

Bioorg Med Chem Lett. 2012 Mar 1;22(5):1908-11. doi: 10.1016/j.bmcl.2012.01.058. Epub 2012 Jan 28.

Abstract

Two new cyclic peptides, dianthins G-H (1 and 2), together with the known dianthin E (3), were isolated from the traditional Chinese medicinal plant Dianthus superbus. The sequences of cyclic peptides 1 and 2 were elucidated as cyclo (-Gly(1)-Pro(2)-Leu(3)-Thr(4)-Leu(5)-Phe(6)-) and cyclo (-Gly(1)-Pro(2)-Val(3)-Thr(4)-Ile(5)-Phe(6)-), on the basis of ESI tandem mass fragmentation analysis, extensive 2D NMR methods and X-ray diffraction. The isolated three compounds all increase proliferation of MC3T3-E1 cells in vitro using MTT method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cell Proliferation / drug effects*
  • Dianthus / chemistry*
  • Mice
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects*
  • Peptides, Cyclic / chemistry*
  • Peptides, Cyclic / isolation & purification
  • Peptides, Cyclic / pharmacology*
  • Plants, Medicinal / chemistry

Substances

  • Peptides, Cyclic