Abstract
A salt tolerant plant, Corydalis heterocarpa has been used as a folk medicine to treat travail and spasm. Recent studies have also reported antioxidant and antiinflammatory activities of compounds isolated from C. heterocarpa. In this study, the protective effect of (2'S)-columbianetin isolated from C. heterocarpa on UVB-induced human keratinocyte (HaCaT) damage was investigated. First, the appropriate energy level of UVB irradiation was determined using MTT and LDH assays. And then the protective effect of (2'S)-columbianetin on UVB induced HaCaT damage was evaluated by measuring; the changes in cell viability, LDH release level, ROS generation, cell cycle arrest and MMP expression levels. Finally, the effect of compound on MAPK and AP-1 signaling pathways were studied to understand the underlying signaling mechanisms. Result demonstrated that the presence of (2'S)-columbianetin suppressed the reactive oxygen species (ROS) generation, cell cycle arrest at sub-G1 phase and down regulation of MMP expression in UVB treated HaCaT cells. Furthermore, stress activated signaling pathways (ASK1-MAPK) and AP-1 signaling pathway were regulated by (2'S)-columbianetin treatment. These results suggest that (2'S)-columbianetin could be effectively used to protect human keratinocytes from UVB induced damage.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Cycle Checkpoints / drug effects
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Cell Cycle Checkpoints / radiation effects
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Cell Line
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Cell Survival / drug effects
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Cell Survival / radiation effects
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Corydalis / chemistry*
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Enzyme Activation / drug effects
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Enzyme Activation / radiation effects
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology*
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Furocoumarins / isolation & purification
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Furocoumarins / pharmacology*
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / radiation effects
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Humans
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Hydrogen Peroxide / metabolism
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Intracellular Space / drug effects
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Intracellular Space / metabolism
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Intracellular Space / radiation effects
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Keratinocytes / cytology
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Keratinocytes / drug effects*
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Keratinocytes / metabolism
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Keratinocytes / radiation effects*
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MAP Kinase Kinase Kinase 5 / metabolism
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Matrix Metalloproteinases / genetics
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Matrix Metalloproteinases / metabolism
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Mitogen-Activated Protein Kinases / metabolism
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Phosphorylation / drug effects
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Phosphorylation / radiation effects
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Radiation-Protective Agents / isolation & purification
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Radiation-Protective Agents / pharmacology*
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Tissue Inhibitor of Metalloproteinases / genetics
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Tissue Inhibitor of Metalloproteinases / metabolism
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Transcription Factor AP-1 / metabolism
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Ultraviolet Rays / adverse effects*
Substances
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Free Radical Scavengers
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Furocoumarins
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Radiation-Protective Agents
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Tissue Inhibitor of Metalloproteinases
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Transcription Factor AP-1
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columbianetin
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Hydrogen Peroxide
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Mitogen-Activated Protein Kinases
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MAP Kinase Kinase Kinase 5
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Matrix Metalloproteinases