A fluorometric method for evaluation of pharmacological activity against intracellular Leishmania amastigotes

Pablo Bilbao-Ramos; Sergio Sifontes-Rodríguez; María Auxiliadora Dea-Ayuela; Francisco Bolás-Fernández

doi:10.1016/j.mimet.2012.01.013

A fluorometric method for evaluation of pharmacological activity against intracellular Leishmania amastigotes

J Microbiol Methods. 2012 Apr;89(1):8-11. doi: 10.1016/j.mimet.2012.01.013. Epub 2012 Jan 28.

Authors

Pablo Bilbao-Ramos¹, Sergio Sifontes-Rodríguez, María Auxiliadora Dea-Ayuela, Francisco Bolás-Fernández

Affiliation

¹ Departamento de Parasitología. Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040-Madrid, Spain.

PMID: 22310031
DOI: 10.1016/j.mimet.2012.01.013

Abstract

In this work a simple and novel method to evaluate the efficacy of compounds on intracellular Leishmania amastigotes by using a fluorometric assay has been developed. The new method is sensitive, easy to perform and scalable for high throughput and therefore it could be validated for screening of new anti-leishmanial agents.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antiprotozoal Agents / pharmacology*
Cell Line
Drug Evaluation, Preclinical / methods
Fluorometry / methods*
High-Throughput Screening Assays / methods
Leishmania / drug effects*
Macrophages / parasitology
Mice
Parasitic Sensitivity Tests / methods
Sensitivity and Specificity

Substances

Antiprotozoal Agents