An aptamer based on-plate microarray for high-throughput insulin detection by MALDI-TOF MS

Chem Commun (Camb). 2012 Mar 11;48(21):2689-91. doi: 10.1039/c2cc17997k. Epub 2012 Feb 3.

Abstract

An aptamer microarray was directly fabricated on a MALDI target plate for high-throughput insulin detection. High sensitivities were observed both in standard solutions (5 ng mL(-1), 0.86 nM) and serum sample (20 ng mL(-1), 3.4 nM). This method shows great promise in the field of biomarker detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Chemistry Techniques, Analytical / methods*
  • Gold / chemistry
  • Humans
  • Insulin / analysis*
  • Metal Nanoparticles / chemistry
  • Metal Nanoparticles / ultrastructure
  • Protein Array Analysis
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

  • Aptamers, Nucleotide
  • Insulin
  • Gold