Abstract
In this study we demonstrate that the photoconvertible monomeric Kikume green-red (mKikGR) protein is suitable to study trafficking of G protein-coupled receptors. Taking mKikGR-tagged mutants of the vasopressin V(2) receptor (V(2)R) as models, we analyzed whether the V(2)R-specific pharmacological chaperone SR121463B influences receptor folding on a co- or post-translational level. Misfolded mKikGR-tagged V(2)Rs were completely photoconverted in the early secretory pathway yielding a red receptor population (already synthesized receptors) and an arising green receptor population (newly synthesized receptors). Trafficking of both receptor populations could be rescued by treatment with SR121463B demonstrating that the substance can act co- and post-translationally.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antidiuretic Hormone Receptor Antagonists
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Color
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HEK293 Cells
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Humans
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism*
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Molecular Chaperones / chemistry
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Molecular Chaperones / metabolism*
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Molecular Sequence Data
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Morpholines / chemistry
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Morpholines / metabolism
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Protein Folding
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Receptors, G-Protein-Coupled / chemistry
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Receptors, G-Protein-Coupled / genetics
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Receptors, G-Protein-Coupled / metabolism*
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Receptors, Vasopressin / chemistry
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Receptors, Vasopressin / genetics
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Receptors, Vasopressin / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Spiro Compounds / chemistry
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Spiro Compounds / metabolism
Substances
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Antidiuretic Hormone Receptor Antagonists
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Luminescent Proteins
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Molecular Chaperones
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Morpholines
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Receptors, G-Protein-Coupled
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Receptors, Vasopressin
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Recombinant Fusion Proteins
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Spiro Compounds
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mKikGR protein