Production and characterization of Clostridium perfringens recombinant β toxoid

Anaerobe. 2012 Jun;18(3):363-5. doi: 10.1016/j.anaerobe.2012.01.004. Epub 2012 Jan 24.

Abstract

In this work, we produced and evaluated a vaccine based on a β toxoid of Clostridium perfringens type C produced in Escherichia coli (rBT). The non-toxic rBT was innocuous for mice and induced 14 IU mL(-1) of β antitoxin in rabbits, complying with the European Pharmacopeia and CFR9 - USDA guidelines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Toxins / genetics
  • Bacterial Vaccines / biosynthesis*
  • Bacterial Vaccines / genetics
  • Bacterial Vaccines / toxicity
  • Clostridium perfringens / immunology*
  • Escherichia coli
  • Mice
  • Mice, Inbred BALB C
  • Rabbits
  • Toxoids / biosynthesis*
  • Toxoids / genetics
  • Toxoids / toxicity
  • Vaccination
  • Vaccines, Synthetic / biosynthesis*
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / toxicity

Substances

  • Bacterial Toxins
  • Bacterial Vaccines
  • Toxoids
  • Vaccines, Synthetic