Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector , evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins.
Keywords: Charged aerosol; ESI; diode array detector; evaporative light scattering detection; high-performance liquid chromatography; mass spectrometry; mobile phase; potodiode array detectors; sapogenins; saponins; ultraviolet.