A selective fluorescent turn-on NIR probe for cysteine

Org Biomol Chem. 2012 Mar 14;10(10):1966-8. doi: 10.1039/c2ob07046d. Epub 2012 Feb 3.

Abstract

A selective and sensitive turn-on fluorescent NIR probe for cysteine has been developed. Cleavage of 2,4-dinitrobenzenesulfonyl (DNBS) with thiols switches the weakly fluorescent aza-BODIPY dye (λ(em) = 734 nm, Φ(f) = 0.03) to a strongly fluorescent species in the NIR region (λ(em) = 755 nm, Φ(f) = 0.14).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzenesulfonates / chemistry
  • Boron Compounds / chemistry*
  • Cysteine / analysis*
  • Fluorescent Dyes / chemistry*
  • Spectrometry, Fluorescence
  • Sulfhydryl Compounds / chemistry

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Benzenesulfonates
  • Boron Compounds
  • Fluorescent Dyes
  • Sulfhydryl Compounds
  • dinitrobenzenesulfonic acid
  • Cysteine