[A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel]

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2011 Nov;27(4):510-2.
[Article in Chinese]

Abstract

Objective: To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.

Methods: DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.

Results: The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.

Conclusion: The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Separation / methods*
  • Female
  • Ganglia, Spinal / cytology*
  • Ion Channel Gating / physiology*
  • Ion Channels
  • Male
  • Neurons / cytology*
  • Neurons / physiology
  • Patch-Clamp Techniques
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Ion Channels