Population-based study of DNA image cytometry as screening method for esophageal cancer

World J Gastroenterol. 2012 Jan 28;18(4):375-82. doi: 10.3748/wjg.v18.i4.375.

Abstract

Aim: To explore the DNA image cytometry (DNA-ICM) technique as a primary screening method for esophageal squamous precancerous lesions.

Methods: This study was designed as a population-based screening study. A total of 582 local residents aged 40 years-69 years were recruited from Linzhou in Henan and Feicheng in Shandong. However, only 452 subjects had results of liquid-based cytology, DNA-ICM and pathology. The sensitivity and specificity of DNA-ICM were calculated and compared with liquid-based cytology in moderate dysplasia or worse.

Results: Sensitivities of DNA-ICM ranging from at least 1 to 4 aneuploid cells were 90.91%, 86.36%, 79.55% and 77.27%, respectively, which were better than that of liquid-based cytology (75%). Specificities of DNA-ICM were 70.83%, 84.07%, 92.65% and 96.81%, but the specificity of liquid-based cytology was 91.91%. The sensitivity and specificity of a combination of liquid-based cytology and DNA-ICM were 84.09% and 85.78%, respectively.

Conclusion: It is possible to use DNA-ICM technique as a primary screening method for esophageal squamous precancerous lesions.

Keywords: Aneuploidy; DNA image cytometry; Esophageal cancer; Precancerous lesions; Screening.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • China
  • Cytodiagnosis / methods
  • DNA*
  • Esophageal Neoplasms / diagnosis*
  • Esophageal Neoplasms / genetics*
  • Esophageal Neoplasms / pathology
  • Female
  • Humans
  • Image Cytometry / methods*
  • Male
  • Mass Screening / methods*
  • Middle Aged
  • Precancerous Conditions / diagnosis*
  • Precancerous Conditions / genetics*
  • Precancerous Conditions / pathology
  • ROC Curve
  • Sensitivity and Specificity

Substances

  • DNA