Flow cytometry of fluorescent proteins

William G Telford; Teresa Hawley; Fedor Subach; Vladislav Verkhusha; Robert G Hawley

doi:10.1016/j.ymeth.2012.01.003

Flow cytometry of fluorescent proteins

Methods. 2012 Jul;57(3):318-30. doi: 10.1016/j.ymeth.2012.01.003. Epub 2012 Jan 24.

Authors

William G Telford¹, Teresa Hawley, Fedor Subach, Vladislav Verkhusha, Robert G Hawley

Affiliation

¹ Experimental Transplantation and Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. telfordw@mail.nih.gov

PMID: 22293036
DOI: 10.1016/j.ymeth.2012.01.003

Abstract

Fluorescent proteins are now a critical tool in all areas of biomedical research. In this article, we review the techniques required to use fluorescent proteins for flow cytometry, concentrating specifically on the excitation and emission requirements for each protein, and the specific equipment required for optimal use.

Published by Elsevier Inc.

Publication types

Review

MeSH terms

Bacterial Proteins / chemistry*
Cells, Cultured
Escherichia coli / genetics
Flow Cytometry / methods*
Fluorescent Dyes
Genetic Vectors
Green Fluorescent Proteins / chemistry*
Humans
Luminescent Proteins / chemistry*
Recombinant Fusion Proteins / chemistry
Red Fluorescent Protein
Spectrometry, Fluorescence

Substances

Bacterial Proteins
Fluorescent Dyes
Luminescent Proteins
Recombinant Fusion Proteins
fluorescent protein 583
yellow fluorescent protein, Bacteria
Green Fluorescent Proteins