Abstract
The application of an HPLC bioactivity profiling/microtiter plate technique in conjunction with microprobe NMR instrumentation and access to the AntiMarin database has led to the isolation of a new 1. In this example, 1 was isolated from a cytotoxic fraction of an extract obtained from marine-derived Streptomyces sp. cultured on Starch Casein Agar (SCA) medium. The 1D and 2D (1)H NMR and ESIMS data obtained from 20 μg of compound 1 fully defined the structure. The known 2 was also isolated and readily dereplicated using this approach.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line, Tumor
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Chromatography, High Pressure Liquid / methods
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Drug Screening Assays, Antitumor / methods*
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Magnetic Resonance Spectroscopy / methods
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Marine Biology
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Mice
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Molecular Structure
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Pyrrolizidine Alkaloids / chemistry
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Pyrrolizidine Alkaloids / isolation & purification*
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Streptomyces / chemistry*
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Sulfur Compounds / chemical synthesis
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Sulfur Compounds / isolation & purification
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Water Microbiology*
Substances
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Pyrrolizidine Alkaloids
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S-methyl 2,4-dihydroxy-6-isopropyl-3,5-dimethylbenzothioate
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Sulfur Compounds
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bohemamine