Abstract
An Achromobacter xylosoxidans strain from the Tripoli central hospital produced a unique metallo-β-lactamase, designated TMB-1, which is related to DIM-1 (62%) and GIM-1 (51%). bla(TMB-1) was embedded in a class 1 integron and located on the chromosome. The TMB-1 β-lactamase has lower k(cat) values than both DIM-1 and GIM-1 with cephalosporins and carbapenems. The K(m) values were more similar to those of GIM-1 than those of DIM-1, with the overall k(cat)/K(m) values being lower than those for GIM-1 and DIM-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Achromobacter denitrificans / enzymology
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Achromobacter denitrificans / genetics*
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Achromobacter denitrificans / isolation & purification
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Amino Acid Sequence
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Anti-Bacterial Agents / administration & dosage
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Anti-Bacterial Agents / therapeutic use
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Carbapenems / administration & dosage
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Carbapenems / therapeutic use
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Cephalosporins / administration & dosage
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Cephalosporins / therapeutic use
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Chromosomes, Bacterial / genetics
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DNA, Bacterial / genetics*
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Gram-Negative Bacterial Infections / drug therapy
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Gram-Negative Bacterial Infections / microbiology*
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Humans
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Integrons / genetics
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Kinetics
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Libya
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Pseudomonas aeruginosa / genetics
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Sequence Alignment
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Sequence Homology, Amino Acid
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beta-Lactamases / genetics*
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beta-Lactamases / isolation & purification
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Carbapenems
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Cephalosporins
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DNA, Bacterial
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beta-Lactamases
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beta-lactamase GIM-1, Pseudomonas aeruginosa