Pyrosequencing-based barcodes for a dye-free multiplex bioassay

Zhiyao Chen; Xiaoying Fu; Xiaodan Zhang; Xiqun Liu; Bingjie Zou; Haiping Wu; Qinxin Song; Jinheng Li; Tomoharu Kajiyama; Hideki Kambara; Guohua Zhou

doi:10.1039/c2cc17618a

Pyrosequencing-based barcodes for a dye-free multiplex bioassay

Chem Commun (Camb). 2012 Feb 28;48(18):2445-7. doi: 10.1039/c2cc17618a. Epub 2012 Jan 23.

Authors

Zhiyao Chen¹, Xiaoying Fu, Xiaodan Zhang, Xiqun Liu, Bingjie Zou, Haiping Wu, Qinxin Song, Jinheng Li, Tomoharu Kajiyama, Hideki Kambara, Guohua Zhou

Affiliation

¹ Department of Pharmacology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, PR China.

PMID: 22267084
DOI: 10.1039/c2cc17618a

Abstract

A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / genetics
Animals
Base Sequence
Biological Assay / methods*
Cyclin-Dependent Kinase 4 / genetics
Mice
Sequence Analysis / methods*
Staining and Labeling

Substances

Actins
Cyclin-Dependent Kinase 4