A Seoul-Fluor-based bioprobe for lipid droplets and its application in image-based high throughput screening

Chem Commun (Camb). 2012 Feb 25;48(17):2331-3. doi: 10.1039/c2cc17496k. Epub 2012 Jan 23.

Abstract

We developed a novel fluorescent bioprobe (SF44) that can specifically visualize the cellular lipid droplets in in vitro and in vivo systems and illustrated the mechanistic rationale of its fluorogenic property. Its application to image-based high throughput screening led us to the identification of a new small-molecule modulator of lipid droplet formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Boron Compounds / chemistry
  • Fatty Acids / chemistry
  • HeLa Cells
  • Heterocyclic Compounds, 4 or More Rings / chemistry*
  • Heterocyclic Compounds, 4 or More Rings / pharmacology
  • High-Throughput Screening Assays
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Lipids / chemistry*
  • Mice
  • Molecular Probes / chemistry*
  • Molecular Probes / pharmacology
  • Oxazines / chemistry

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fatty Acids
  • Heterocyclic Compounds, 4 or More Rings
  • Lipids
  • Molecular Probes
  • Oxazines
  • SF44 compound
  • nile red