A C-di-GMP-proflavine-hemin supramolecular complex has peroxidase activity--implication for a simple colorimetric detection

Mol Biosyst. 2012 Mar;8(3):726-9. doi: 10.1039/c2mb05430b. Epub 2012 Jan 18.

Abstract

Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙(+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Benzothiazoles / chemistry
  • Benzothiazoles / metabolism
  • Colorimetry
  • Cyclic GMP / analogs & derivatives*
  • Cyclic GMP / chemistry
  • Cyclic GMP / metabolism
  • Hemin
  • Oxidation-Reduction
  • Peroxidase / chemistry*
  • Peroxidase / metabolism
  • Proflavine / chemistry*
  • Proflavine / metabolism
  • Sulfonic Acids / chemistry
  • Sulfonic Acids / metabolism

Substances

  • Benzothiazoles
  • Sulfonic Acids
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • bis(3',5')-cyclic diguanylic acid
  • Hemin
  • Proflavine
  • Peroxidase
  • Cyclic GMP