Abstract
Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙(+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Benzothiazoles / chemistry
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Benzothiazoles / metabolism
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Colorimetry
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Cyclic GMP / analogs & derivatives*
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Cyclic GMP / chemistry
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Cyclic GMP / metabolism
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Hemin
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Oxidation-Reduction
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Peroxidase / chemistry*
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Peroxidase / metabolism
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Proflavine / chemistry*
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Proflavine / metabolism
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Sulfonic Acids / chemistry
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Sulfonic Acids / metabolism
Substances
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Benzothiazoles
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Sulfonic Acids
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2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
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bis(3',5')-cyclic diguanylic acid
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Hemin
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Proflavine
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Peroxidase
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Cyclic GMP