Preparation of free, soluble conjugate, and insoluble-bound phenolic compounds from peels of rambutan (Nephelium lappaceum) and evaluation of antioxidant activities in vitro

J Food Sci. 2012 Feb;77(2):C198-204. doi: 10.1111/j.1750-3841.2011.02548.x. Epub 2012 Jan 17.

Abstract

The soluble phenolic compounds of rambutan peels (RP) were extracted by microwave-assisted extraction (MAE) and the operating parameters were optimized. The optimal conditions obtained were ethanol concentration of 80.85%, extraction time of 58.39 s, and the ratio of liquid to solid of 24.51:1. The soluble phenolic content by MAE was 213.76 mg GAE/g DW. The free, soluble conjugate, and insoluble-boaund phenolic compounds were prepared by alkaline hydrolysis, and the contents of 3 fractions were 185.12, 27.98 and 9.37 mg GAE/g DW, respectively. The contents of syringic acid and p-coumaric acid were high in the free fraction, showing 16.86 and 19.44 mg/g DW, and the soluble conjugate and insoluble-bound phenolics were mainly composed of gallic acid and caffeic acid. Furthermore, the antioxidant activities of 3 fractions were evaluated in 5 model systems. Results indicated that the free fraction had high antioxidant activities, compared with the soluble conjugate and insoluble-bound fractions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Caffeic Acids / analysis
  • Coumaric Acids / analysis
  • Gallic Acid / analogs & derivatives
  • Gallic Acid / analysis
  • Lipid Peroxidation
  • Phenols / pharmacology*
  • Plant Extracts / pharmacology*
  • Propionates
  • Sapindaceae / chemistry*

Substances

  • Antioxidants
  • Caffeic Acids
  • Coumaric Acids
  • Phenols
  • Plant Extracts
  • Propionates
  • Gallic Acid
  • syringic acid
  • p-coumaric acid
  • caffeic acid