Abstract
Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Drug Resistance, Bacterial*
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Genes, Bacterial
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Genetics, Microbial / methods*
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Gentamicins / pharmacology*
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Mutagenesis, Insertional / methods*
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Selection, Genetic*
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Treponema denticola / genetics*
Substances
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Anti-Bacterial Agents
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Gentamicins