Discovery of an entropically-driven small molecule streptavidin binder from nucleic acid-encoded libraries

Jean-Pierre Daguer; Mihai Ciobanu; Sofia Barluenga; Nicolas Winssinger

doi:10.1039/c2ob06880j

Discovery of an entropically-driven small molecule streptavidin binder from nucleic acid-encoded libraries

Org Biomol Chem. 2012 Feb 28;10(8):1502-5. doi: 10.1039/c2ob06880j. Epub 2012 Jan 12.

Authors

Jean-Pierre Daguer¹, Mihai Ciobanu, Sofia Barluenga, Nicolas Winssinger

Affiliation

¹ Institut de Science et Ingenierie Supramoleculaires (ISIS), Université de Strasbourg-CNRS (UMR 7006), 8 allee Gaspard Monge, 67000 Strasbourg, France.

PMID: 22237952
DOI: 10.1039/c2ob06880j

Abstract

Dehydrocholic acid was identified as a selective streptavidin binder from a PNA-tagged library. Isothermal calorimetry titration measurements showed this interaction to be entropically driven. Peptides tagged with dehydrocholic acid can be captured on a streptavidin resin and released under thermal conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Entropy*
Molecular Structure
Nucleic Acids / chemistry*
Peptides / chemistry
Streptavidin / chemistry*

Substances

Nucleic Acids
Peptides
Streptavidin