FRET spectral unmixing: a ratiometric fluorescent nanoprobe for hypochlorite

Chem Commun (Camb). 2012 Mar 21;48(24):2949-51. doi: 10.1039/c2cc17617c. Epub 2012 Jan 12.

Abstract

A specific ratiometric nanoprobe for hypochlorite was constructed as a paradigm of FRET spectral unmixing. The separation of FRET pairs' emissions reaches 175 nm, which ensures that the FRET probing is more accurate. This new nanoprobe shows high selectivity and potential in biological systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbocyanines / administration & dosage
  • Carbocyanines / chemistry
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / administration & dosage
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Hypochlorous Acid / analysis*
  • Hypochlorous Acid / chemistry
  • Microscopy, Confocal
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry
  • Rhodamines / administration & dosage
  • Rhodamines / chemistry
  • Silicon Dioxide / administration & dosage
  • Silicon Dioxide / chemistry

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • Rhodamines
  • Hypochlorous Acid
  • Silicon Dioxide
  • rhodamine B