Two-temperature stage biphasic CO2-H2O pretreatment of lignocellulosic biomass at high solid loadings

Biotechnol Bioeng. 2012 Jun;109(6):1499-507. doi: 10.1002/bit.24417. Epub 2012 Jan 7.

Abstract

Most biomass pretreatment processes for monosaccharide production are run at low-solid concentration (<10 wt%) and use significant amounts of chemical catalysts. Biphasic CO(2) -H(2) O mixtures could provide a more sustainable pretreatment medium while using high-solid contents. Using a stirred reactor for high solids (40 wt%, biomass water mixture) biphasic CO(2)-H(2) O pretreatment of lignocellulosic biomass allowed us to explore the effects of particle size and mixing on mixed hardwood and switchgrass pretreatment. Subsequently, a two-temperature stage pretreatment was introduced. After optimization, a short high-temperature stage at 210°C (16 min for hardwood and 1 min for switchgrass) was followed by a long low-temperature stage at 160°C for 60 min. Glucan to glucose conversion yields of 83% for hardwood and 80% for switchgrass were obtained. Total molar sugar yields of 65% and 55% were obtained for wood and switchgrass, respectively, which consisted of a 10% points improvement over those obtained during our previous study despite a 10-fold increase in particle size. These yields are similar to those obtained with other major pretreatment technologies for wood and within 10% of major technologies for switchgrass despite the absence of chemical catalysts, the use of large particles (0.95 cm) and high solid contents (40 wt%).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomass*
  • Carbohydrates / isolation & purification*
  • Carbon Dioxide / chemistry*
  • Chromatography, Supercritical Fluid
  • Hot Temperature
  • Lignin / chemistry*
  • Panicum / chemistry
  • Water / chemistry*
  • Wood / chemistry

Substances

  • Carbohydrates
  • Water
  • lignocellulose
  • Carbon Dioxide
  • Lignin