Abstract
An alternative method for the cultivation of Lawsonia intracellularis, an obligate intracellular bacterium and the causative agent of proliferative enteropathy, was developed using an Original Space Bag inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% nitrogen. The flexibility of this protocol allows the testing of various environmental conditions for static cultivation of this bacterium and the development of diagnostic techniques.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacteriological Techniques / methods*
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Cell Culture Techniques / methods
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Cell Line
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Desulfovibrionaceae Infections / microbiology
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Desulfovibrionaceae Infections / veterinary
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Fibroblasts / microbiology
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Gastrointestinal Diseases / microbiology
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Gastrointestinal Diseases / veterinary
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Lawsonia Bacteria / growth & development*
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Lawsonia Bacteria / isolation & purification
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Mice
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Swine