New examination of the performance of the MetaMax I metabolic analyser with the Douglas-bag technique

Scand J Clin Lab Invest. 2012 Apr;72(2):158-68. doi: 10.3109/00365513.2011.649013. Epub 2012 Jan 4.

Abstract

Purpose: To examine the performance of the MetaMax® I metabolic analyser of Cortex Biophysik, running a recent version of the software (MetaSoft v. 1.11.05), using the Douglas-bag method as reference.

Experiments: First, 182 measurements of the maximal O(2) uptake on school children were analysed both by a former version of the software (MetaMax Analysis) and by a recent version (MetaSoft). In further experiments, seven grown-up subjects of different fitness levels cycled for 5 min at constant powers between 50 and 350 W while the O(2) uptake was measured simultaneously by the MetaMax I and the Douglas-bag method during the last minute of altogether 39 exercises.

Results: The calculated maximal O(2) uptake of the 182 school children was on average 3% lower when the data were reanalysed by MetaSoft than when analysed by the former version of the software. There was in addition a 2% variation. In further experiments on grown-up subjects the O(2) uptake reported by the MetaMax I did not differ from that reported by the Douglas-bag system when data for all subjects were pooled, and the random error was 4%. However, the relationships differed slightly between the subjects (p = 0.005), and consequently the random error within each subject was 2-3%. The respiratory exchange ratio (R-value) reported by the MetaMax I differed systematically from that of the control method. However, a reliable R-value could be calculated from the instrument's raw data.

Conclusions: Our data suggest that the MetaMax I running MetaSoft reports unbiased values of the O(2) uptake and with moderate random error.

MeSH terms

  • Adult
  • Biophysics
  • Child
  • Female
  • Humans
  • Male
  • Metabolism*
  • Middle Aged
  • Oxygen / metabolism

Substances

  • Oxygen