Innovative collagen nano-hydroxyapatite scaffolds offer a highly efficient non-viral gene delivery platform for stem cell-mediated bone formation

Caroline M Curtin; Gráinne M Cunniffe; Frank G Lyons; Kazuhisa Bessho; Glenn R Dickson; Garry P Duffy; Fergal J O'Brien

doi:10.1002/adma.201103828

Innovative collagen nano-hydroxyapatite scaffolds offer a highly efficient non-viral gene delivery platform for stem cell-mediated bone formation

Adv Mater. 2012 Feb 7;24(6):749-54. doi: 10.1002/adma.201103828. Epub 2012 Jan 2.

Authors

Caroline M Curtin¹, Gráinne M Cunniffe, Frank G Lyons, Kazuhisa Bessho, Glenn R Dickson, Garry P Duffy, Fergal J O'Brien

Affiliation

¹ Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland.

PMID: 22213347
DOI: 10.1002/adma.201103828

Abstract

The ability of nano-hydroxyapatite (nHA) particles developed in-house to act as non-viral delivery vectors is assessed. These nHA particles are combined with collagen to yield bioactive, biodegradable collagen nano-hydroxyapatite (coll-nHA) scaffolds. Their ability to act as gene-activated matrices for BMP2 delivery is demonstrated with successful transfection of mesenchymal stem cells (MSCs) resulting in high calcium production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Morphogenetic Proteins / genetics
Cell Line
Collagen / chemistry
Collagen / metabolism*
Durapatite / metabolism*
Humans
Mesenchymal Stem Cells / cytology*
Nanomedicine
Nanostructures / chemistry*
Osteogenesis*
Rats
Tissue Scaffolds / chemistry*
Transfection / methods*

Substances

Bone Morphogenetic Proteins
Collagen
Durapatite