Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella Bacteremia

J Clin Microbiol. 2012 Mar;50(3):1122-4. doi: 10.1128/JCM.05121-11. Epub 2012 Jan 11.

Abstract

Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.

Publication types

  • Case Reports
  • Evaluation Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacteremia / diagnosis*
  • Bacteremia / microbiology
  • Bacteriological Techniques / methods*
  • Humans
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Polymerase Chain Reaction / methods*
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Salmonella Infections / diagnosis*
  • Salmonella Infections / microbiology
  • Salmonella enterica / classification*
  • Salmonella enterica / genetics
  • Salmonella enterica / isolation & purification*
  • Time Factors
  • Transition Temperature

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S