Abstract
We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla(KPC) fragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla(KPC) is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Base Sequence / genetics*
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Carbapenems / pharmacology*
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DNA Transposable Elements
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Escherichia coli / genetics
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Humans
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Klebsiella Infections / microbiology
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Multilocus Sequence Typing
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Polymerase Chain Reaction
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Sequence Deletion
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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DNA Transposable Elements
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beta-Lactamases