F-actin staining of Drosophila testes

Cold Spring Harb Protoc. 2012 Jan 1;2012(1):105-6. doi: 10.1101/pdb.prot067348.

Abstract

Preparations of Drosophila testes fixed with paraformaldehyde can be stained for F-actin according to the protocol described here. This staining procedure is particularly suitable for staining the male fusome and the cytokinetic contractile ring.

MeSH terms

  • Actins / analysis*
  • Animals
  • Drosophila / anatomy & histology*
  • Fixatives / metabolism
  • Formaldehyde / metabolism
  • Male
  • Polymers / metabolism
  • Staining and Labeling / methods*
  • Testis / anatomy & histology
  • Testis / chemistry
  • Tissue Fixation / methods

Substances

  • Actins
  • Fixatives
  • Polymers
  • Formaldehyde
  • paraform