The effect of base-pair sequence on electrochemically driven denaturation

Robert P Johnson; Rachel Gao; Tom Brown; Philip N Bartlett

doi:10.1016/j.bioelechem.2011.11.003

The effect of base-pair sequence on electrochemically driven denaturation

Bioelectrochemistry. 2012 Jun:85:7-13. doi: 10.1016/j.bioelechem.2011.11.003. Epub 2011 Dec 2.

Authors

Robert P Johnson¹, Rachel Gao, Tom Brown, Philip N Bartlett

Affiliation

¹ School of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom.

PMID: 22192638
DOI: 10.1016/j.bioelechem.2011.11.003

Abstract

Application of a voltage ramp can result in denaturation of dsDNA and strand separation. We show that the potential at which half of the surface immobilised duplexes denature (the melting potential, E(m)) directly correlates with the calculated nearest neighbour and experimental melting temperatures; T(m), for a duplex is solution. The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pairing
Base Sequence
DNA / chemistry*
DNA Probes
Drug Design
Electrochemical Techniques*
Nucleic Acid Conformation
Nucleic Acid Denaturation*

Substances

DNA Probes
DNA