Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats

Transgenic Res. 2012 Aug;21(4):895-9. doi: 10.1007/s11248-011-9575-x. Epub 2011 Dec 20.

Abstract

The feasibility of genetically engineering soybean seed coats to divert metabolism towards the production of novel biochemicals was tested. The genes phbA, phbB, phbC from Ralstonia eutropha each under the control of the seed coat peroxidase promoter were introduced into soybean and the production of polyhydroxybutyrate (PHB) was assayed. The analysis of seed coats arising from 4 independent transformation events demonstrated that PHB was produced at a mean of 0.12% seed coat dried weight with individual values up to 0.36%. These values demonstrate that it is possible to metabolically engineer soybean seed coats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Cupriavidus necator / genetics
  • Glycine max* / genetics
  • Glycine max* / metabolism
  • Hydroxybutyrates* / chemistry
  • Metabolic Engineering
  • Plants, Genetically Modified* / genetics
  • Plants, Genetically Modified* / metabolism
  • Seeds / genetics
  • Seeds / metabolism

Substances

  • Bacterial Proteins
  • Hydroxybutyrates