Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter

Mami Tojino; Masako Mori; Maria Carmelita Z Kasuya; Kenichi Hatanaka; Atsushi Kawaguchi; Kyosuke Nagata; Takashi Shirai; Mamoru Mizuno

doi:10.1016/j.bmcl.2011.11.057

Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter

Bioorg Med Chem Lett. 2012 Jan 15;22(2):1251-4. doi: 10.1016/j.bmcl.2011.11.057. Epub 2011 Nov 22.

Authors

Mami Tojino¹, Masako Mori, Maria Carmelita Z Kasuya, Kenichi Hatanaka, Atsushi Kawaguchi, Kyosuke Nagata, Takashi Shirai, Mamoru Mizuno

Affiliation

¹ Laboratory of Glyco-organic Chemistry, The Noguchi Institute, 1-8-1 Kaga, Itabashi-ku, Tokyo 173-0003, Japan.

PMID: 22177082
DOI: 10.1016/j.bmcl.2011.11.057

Abstract

The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Siaα2-3Galβ1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorous affinity and simply assessed by dot blot method using the interaction of carbohydrate- with proteins which recognize sialic acid such as virus membrane proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cricetinae
Influenza A virus / chemistry*
Membranes, Artificial
Oligosaccharides / chemistry*
Polyethylene Glycols / chemistry
Polytetrafluoroethylene / chemistry*

Substances

Membranes, Artificial
Oligosaccharides
Polyethylene Glycols
Polytetrafluoroethylene