Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam

P Bianchini; A Diaspro

doi:10.1111/j.1365-2818.2011.03577.x

Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam

J Microsc. 2012 Mar;245(3):225-8. doi: 10.1111/j.1365-2818.2011.03577.x. Epub 2011 Dec 15.

Authors

P Bianchini¹, A Diaspro

Affiliation

¹ Department of Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy.

PMID: 22171566
DOI: 10.1111/j.1365-2818.2011.03577.x

Abstract

In this paper we report stimulated emission depletion (STED) and two-photon excitation (2PE) fluorescence microscopy with continuous wave (CW) laser beam using a new generation laser scanning confocal microscope equipped for STED-CW (TCS STED-CW, Leica Microsystems, Mannheim, Germany). We show the possibility to achieve CW-2PE with the very same beam used for STED-CW. This feature extends the performance of the microscope allowing multimodal imaging (CW-2PE, STED-CW, confocal).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Endothelial Cells / ultrastructure*
Microscopy, Confocal / instrumentation
Microscopy, Confocal / methods*
Microscopy, Fluorescence / instrumentation
Microscopy, Fluorescence / methods*
Pulmonary Artery / ultrastructure