Abstract
By bioguided fractionation of the hexane extract of Commiphora erythraea resin we isolated four furanosesquiterpenoids that were tested for their protective activity against oxidative stress. Furanodienone and 1,10(15)-furanogermacra-dien-6-ones showed to be potent inhibitors of lipid peroxidation (IC(50) of -0.087 μM), being more active than the methoxylated analogues. Furthermore, using BV2 microglial cells, we found that furanodienone from C. erythraea is able to counteract LPS-induced cell death and decrease LPS-induced NO generation thus protecting microglial cells from LPS-induced cytotoxicity. Finally, docking studies were undertaken to gain insight into the possible binding mode of the isolated compounds at 5-LOX binding site.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Death / drug effects
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Cell Line
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Commiphora / chemistry*
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Humans
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Inhibitory Concentration 50
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Lipid Peroxidation / drug effects
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Lipopolysaccharides / pharmacology
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Mice
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Microglia / cytology
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Microglia / drug effects
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Microglia / metabolism
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Models, Molecular
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Oxidative Stress / drug effects*
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Plant Extracts / pharmacology
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Protective Agents / chemistry
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Protective Agents / pharmacology*
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Reactive Nitrogen Species / metabolism
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Resins, Plant / chemistry
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Resins, Plant / isolation & purification
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Resins, Plant / pharmacology*
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Solutions
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Stereoisomerism
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Thermodynamics
Substances
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Lipopolysaccharides
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Plant Extracts
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Protective Agents
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Reactive Nitrogen Species
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Resins, Plant
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Solutions