Bioorthogonal reaction pairs enable simultaneous, selective, multi-target imaging

Angew Chem Int Ed Engl. 2012 Jan 23;51(4):920-2. doi: 10.1002/anie.201104389. Epub 2011 Dec 12.

Abstract

Mutually orthogonal tetrazine–transcyclooctene and azide–cyclooctyne cycloaddition reactions were used simultaneously for the bioorthogonal labeling of two different live cell populations in the same culture. These small-molecule probes show good chemical reactivity and can be readily incorporated into biological systems.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alkynes / chemistry
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / pharmacology
  • Antibodies, Monoclonal, Humanized / chemistry
  • Antibodies, Monoclonal, Humanized / pharmacology
  • Azides / chemistry
  • Cell Line, Tumor
  • Cetuximab
  • Coculture Techniques
  • Contrast Media / chemistry*
  • Contrast Media / pharmacology
  • ErbB Receptors / chemistry
  • ErbB Receptors / metabolism
  • Fluorescent Dyes / chemistry
  • Humans
  • Microscopy, Fluorescence
  • Receptor, ErbB-2 / chemistry
  • Receptor, ErbB-2 / metabolism
  • Trastuzumab

Substances

  • Alkynes
  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Humanized
  • Azides
  • Contrast Media
  • Fluorescent Dyes
  • ERBB2 protein, human
  • ErbB Receptors
  • Receptor, ErbB-2
  • Trastuzumab
  • Cetuximab