Neuromuscular activity of Bothrops alcatraz snake venom in chick biventer cervicis preparations

Toxicon. 2012 Feb;59(2):294-9. doi: 10.1016/j.toxicon.2011.11.011. Epub 2011 Dec 1.

Abstract

Venom (10-100 μg/ml) from Bothrops alcatraz, a pitviper from the Alcatrazes Archipelago off the coast of southeastern Brazil, caused progressive, irreversible neuromuscular blockade in chick isolated biventer cervicis preparations. The venom also inhibited contractures to exogenous ACh (110 μM) and KCl (20 mM), caused myofiber damage and increased creatine kinase release. Commercial bothropic antivenom raised against mainland Bothrops species neutralized the neuromuscular activity, depending on the venom concentration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / adverse effects
  • Animals
  • Antivenins / pharmacology
  • Bothrops*
  • Brazil
  • Chickens
  • Creatine Kinase / metabolism
  • Male
  • Muscle, Skeletal / drug effects
  • Myofibrils / drug effects
  • Myofibrils / pathology
  • Neuromuscular Blockade*
  • Phospholipases A / metabolism
  • Phrenic Nerve
  • Potassium Chloride / adverse effects
  • Receptors, Nicotinic / metabolism
  • Snake Venoms / toxicity*

Substances

  • Antivenins
  • Receptors, Nicotinic
  • Snake Venoms
  • Potassium Chloride
  • Creatine Kinase
  • Phospholipases A
  • Acetylcholine