This study evaluates the sensitivity of the surface albedo simulated by the Integrated Biosphere Simulator (IBIS) to a set of Amazonian tropical rainforest canopy architectural and optical parameters. The parameters tested in this study are the orientation and reflectance of the leaves of upper and lower canopies in the visible (VIS) and near-infrared (NIR) spectral bands. The results are evaluated against albedo measurements taken above the K34 site at the INPA (Instituto Nacional de Pesquisas da Amazônia) Cuieiras Biological Reserve. The sensitivity analysis indicates a strong response to the upper canopy leaves orientation (χup) and to the reflectivity in the near-infrared spectral band (ρNIR,up), a smaller sensitivity to the reflectivity in the visible spectral band (ρVIS,up) and no sensitivity at all to the lower canopy parameters, which is consistent with the canopy structure. The combination of parameters that minimized the Root Mean Square Error and mean relative error are χup = 0.86, ρVIS,up = 0.062 and ρNIR,up = 0.275. The parameterizations performed resulted in successful simulations of tropical rainforest albedo by IBIS, indicating its potential to simulate the canopy radiative transfer for narrow spectral bands and permitting close comparison with remote sensing products.