Colorimetric detection of apoptosis based on caspase-3 activity assay using unmodified gold nanoparticles

Yuliang Pan; Manli Guo; Zhou Nie; Yan Huang; Ying Peng; Afang Liu; Meng Qing; Shouzhuo Yao

doi:10.1039/c1cc15407a

Colorimetric detection of apoptosis based on caspase-3 activity assay using unmodified gold nanoparticles

Chem Commun (Camb). 2012 Jan 25;48(7):997-9. doi: 10.1039/c1cc15407a. Epub 2011 Dec 5.

Authors

Yuliang Pan¹, Manli Guo, Zhou Nie, Yan Huang, Ying Peng, Afang Liu, Meng Qing, Shouzhuo Yao

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.

PMID: 22143383
DOI: 10.1039/c1cc15407a

Abstract

A new label-free method for the detection of apoptosis was proposed based on colorimetric assay of caspase-3 activity using an unlabeled Asp-Glu-Val-Asp (DEVD)-containing peptide substrate and unmodified gold nanoparticles (AuNPs).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Apoptosis*
Caspase 3 / metabolism*
Colorimetry / methods
Gold / chemistry*
Gold / metabolism
Humans
Jurkat Cells
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Peptides / chemistry
Peptides / metabolism*

Substances

Peptides
Gold
Caspase 3