Green fluorescent protein-labeled monitoring tool to quantify conjugative plasmid transfer between Gram-positive and Gram-negative bacteria

Karsten Arends; Katarzyna Schiwon; Türkan Sakinc; Johannes Hübner; Elisabeth Grohmann

doi:10.1128/AEM.05578-11

Green fluorescent protein-labeled monitoring tool to quantify conjugative plasmid transfer between Gram-positive and Gram-negative bacteria

Appl Environ Microbiol. 2012 Feb;78(3):895-9. doi: 10.1128/AEM.05578-11. Epub 2011 Dec 2.

Authors

Karsten Arends¹, Katarzyna Schiwon, Türkan Sakinc, Johannes Hübner, Elisabeth Grohmann

Affiliation

¹ Department of Environmental Microbiology/Genetics, University of Technology, Berlin, Germany.

Abstract

On the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positive Enterococcus faecalis and Gram-negative Escherichia coli. Furthermore, retromobilization of the GFP-tagged monitoring tool was shown from E. faecalis OG1X into the clinical isolate E. faecalis T9.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Conjugation, Genetic*
Enterococcus faecalis / genetics*
Escherichia coli / genetics*
Gene Transfer, Horizontal*
Genes, Reporter
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Plasmids
Staining and Labeling

Substances

Green Fluorescent Proteins